APP antibody (pThr743)

Catalog No. ABIN1531173

This Rabbit Polyclonal antibody specifically detects APP in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for APP antibody (pThr743) (ABIN1531173)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-APP Antibody

Binding Specificity: AA 711-760, pThr743

Specificity:

Amyloid beta A4 (Phospho-Thr743/668) Antibody detects endogenous levels of Amyloid beta A4 only when phosphorylated at Thr743/668.

PhosphorylationH:T743 M:T743 R:T743

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Amyloid beta A4 around the phosphorylation site of Thr743/668.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

Comment:

Unigene-Number: Hs.434980 (NCBI Gene Symbol: APP)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Background:

Synonyms: A4, ABPP, AD1, APP, APPI, Alzheimer's disease amyloid protein, Amyloid beta A4 protein precursor, Beta-amyloid protein 40, Beta-amyloid protein 42, C31, C83, CVAP, Cerebral vascular amyloid peptide, Gamma-CTF(50), Gamma-CTF(57), Gamma-CTF(59), P3(40), P3(

NCBI Gene Symbol: APP

Molecular Weight: 86 kDa

Gene ID: 351

OMIM: 104300

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour