NFKBIB antibody (pSer23)

Catalog No. ABIN1531183

The Rabbit Polyclonal anti-NFKBIB antibody is suitable to detect NFKBIB in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for NFKBIB antibody (pSer23) (ABIN1531183)

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-NFKBIB Antibody

Binding Specificity: AA 8-57, pSer23

Purpose: IκB-β (phospho Ser23) Antibody

Specificity: Phospho-IκB-β (S23) Polyclonal Antibody detects endogenous levels of IκB-β protein only when phosphorylated at S23.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human IkappaB-beta around the phosphorylation site of Ser23. AA range:8-57

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for NFKBIB

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Background:

Synonyms: NFKBIB, IKBB, TRIP9, NF-kappa-B inhibitor beta, NF-kappa-BIB, I-kappa-B-beta, IkB-B, IkB-beta, IkappaBbeta, Thyroid receptor-interacting protein 9, TR-interacting protein 9, TRIP-9

Background: The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011],

Gene Name: NFKBIB

Protein Name: NF-kappa-B inhibitor beta

Molecular Weight: 37 kDa

Gene ID: 4793

OMIM: 604495

UniProt: Q15653

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Maintenance of Protein Location, Toll-Like Receptors Cascades