Caspase 6 antibody (pSer257)

Catalog No. ABIN1531214

The Rabbit Polyclonal anti-Caspase 6 antibody (ABIN1531214) specifically detects Caspase 6 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Caspase 6 antibody (pSer257) (ABIN1531214)

Target: Caspase 6 (CASP6) (Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Caspase 6 Antibody

Binding Specificity: AA 223-272, pSer257

Purpose: Caspase-6 (phospho Ser257) Antibody

Specificity: Phospho-Caspase-6 (S257) Polyclonal Antibody detects endogenous levels of Caspase-6 protein only when phosphorylated at S257.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 6 around the phosphorylation site of Ser257. AA range:223-272

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Caspase 6

Target: Caspase 6 (CASP6) (Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6))

Alternative Name: Caspase 6

Background:

Synonyms: CASP6, MCH2, Caspase-6, CASP-6, Apoptotic protease Mch-2

Background: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Oct 2015],

Gene Name: CASP6

Protein Name: Caspase6

Molecular Weight: 33 kDa

Gene ID: 839

OMIM: 601532

UniProt: P55212

Pathways: Apoptosis, Caspase Cascade in Apoptosis