KSR1 antibody (pSer392)

Catalog No. ABIN1531231

The Rabbit Polyclonal anti-KSR1 antibody is suitable to detect KSR1 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for KSR1 antibody (pSer392) (ABIN1531231)

Target: KSR1 (Kinase Suppressor of Ras 1 (KSR1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KSR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-KSR1 Antibody

Binding Specificity: AA 358-407, pSer392

Purpose: Ksr-1 (phospho Ser392) Antibody

Specificity: Phospho-Ksr-1 (S392) Polyclonal Antibody detects endogenous levels of Ksr-1 protein only when phosphorylated at S392.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human KSR around the phosphorylation site of Ser392. AA range:358-407

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for KSR1

Target: KSR1 (Kinase Suppressor of Ras 1 (KSR1))

Alternative Name: KSR

Background:

Synonyms: KSR1, KSR, Kinase suppressor of Ras 1

Background: caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,function:Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.,PTM:Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,subcellular location:In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.,subunit:Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner (By similarity). The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization.,

Gene Name: KSR1

Protein Name: Kinase suppressor of Ras 1

Molecular Weight: 102 kDa

Gene ID: 8844

OMIM: 601132

UniProt: Q8IVT5