RUNX1 antibody (pSer276)

Catalog No. ABIN1531257

This Rabbit Polyclonal antibody specifically detects RUNX1 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RUNX1 antibody (pSer276) (ABIN1531257)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RUNX1 Antibody

Binding Specificity: AA 242-291, pSer276

Purpose: RUNX1 (phospho Ser249) Antibody

Specificity: Phospho-RUNX1 (S249) Polyclonal Antibody detects endogenous levels of RUNX1 protein only when phosphorylated at S249.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human AML1 around the phosphorylation site of Ser276. AA range:242-291

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: AML1

Background:

Synonyms: RUNX1, AML1, CBFA2, Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, CBF-alpha-2, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, PEA2-alpha B, PEBP2-alpha

Background: Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters.The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Gene Name: RUNX1

Protein Name: Runt-related transcription factor 1

Molecular Weight: 48 kDa

Gene ID: 861

OMIM: 151385

UniProt: Q01196