Cyclin D1 antibody (pThr286)

Catalog No. ABIN1531265

This anti-Cyclin D1 antibody is a Rabbit Polyclonal antibody detecting Cyclin D1 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for Cyclin D1 antibody (pThr286) (ABIN1531265)

Target: Cyclin D1 (CCND1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Cyclin D1 Antibody

Binding Specificity: AA 246-295, pThr286

Specificity:

Cyclin D1 (Phospho-Thr286) Antibody detects endogenous levels of Cyclin D1 only when phosphorylated at Thr286.

PhosphorylationH:T286 M:T286 R:T286

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin D1 around the phosphorylation site of Thr286.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:1000

Comment:

Unigene-Number: Hs.523852 (NCBI Gene Symbol: CCND1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: Cyclin D1

Background:

Synonyms: BCL-1 oncogene, BCL1, cyclin D1, CYL-1, G1/S-specific cyclin D1, PRAD1, PRAD1 oncogene

NCBI Gene Symbol: CCND1

Molecular Weight: 33 kDa

Gene ID: 595

OMIM: 168461

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling