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Cyclin D1 antibody (pThr286)

The Rabbit Polyclonal anti-Cyclin D1 antibody (ABIN1531265) specifically detects Cyclin D1 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Western blot analysis of extracts from Jurkat cells treated with EGF 200ng/ml 30', using Cyclin D1 (Phospho-Thr286) Antibody. (Cyclin D1 antibody (pThr286))

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Catalog No. ABIN1531265

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Quick Overview for Cyclin D1 antibody (pThr286) (ABIN1531265)

Target

See all Cyclin D1 (CCND1) Antibodies

Cyclin D1 (CCND1)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Cyclin D1 antibody is un-conjugated

Application

Western Blotting (WB), ELISA

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AA 246-295, pThr286

Specificity

Cyclin D1 (Phospho-Thr286) Antibody detects endogenous levels of Cyclin D1 only when phosphorylated at Thr286.
PhosphorylationH:T286 M:T286 R:T286

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity

> 95 %

Immunogen

The antiserum was produced against synthesized peptide derived from human Cyclin D1 around the phosphorylation site of Thr286.

Isotype

IgG
anti-Cyclin D1 (CCND1) (AA 61-110) antibody
CCND1 Reactivity: Human, Mouse, Rat, Cow, Rabbit, Sheep WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Cyclin D1 (CCND1) (C-Term) antibody
CCND1 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Cyclin D1 (CCND1) (AA 68-97) antibody
CCND1 Reactivity: Human, Mouse WB, IF, IHC (p) Host: Rabbit Polyclonal RB8066 unconjugated

anti-Cyclin D1 (CCND1) (AA 167-295) antibody
CCND1 Reactivity: Human IHC, ELISA, FACS, ICC Host: Mouse Monoclonal 3B6G3 unconjugated

anti-Cyclin D1 (CCND1) (AA 1-295) antibody
CCND1 Reactivity: Human, Mouse WB, IHC, ELISA, FACS Host: Mouse Monoclonal 1F7F5 unconjugated

anti-Cyclin D1 (CCND1) (C-Term) antibody
CCND1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Cyclin D1 (CCND1) antibody
KD Validated CCND1 Reactivity: Human WB, FACS Host: Rabbit Monoclonal 23GB735 unconjugated Recombinant Antibody

anti-Cyclin D1 (CCND1) (C-Term) antibody
CCND1 Reactivity: Human WB, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Cyclin D1 (CCND1) antibody
CCND1 Reactivity: Human, Mouse, Rat, Monkey WB, IHC, FACS, IF, StM Host: Mouse Monoclonal DCS-6 unconjugated

anti-Cyclin D1 (CCND1) (AA 1-295) antibody
CCND1 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 1:500~1:1000 ELISA: 1:1000

Comment

Unigene-Number: Hs.523852 (NCBI Gene Symbol: CCND1)

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Stable at -20°C for at least 1 year.

Expiry Date

12 months
Target

Cyclin D1 (CCND1)

Alternative Name

Cyclin D1

Background

Synonyms: BCL-1 oncogene, BCL1, cyclin D1, CYL-1, G1/S-specific cyclin D1, PRAD1, PRAD1 oncogene
NCBI Gene Symbol: CCND1

Molecular Weight

33 kDa

Gene ID

595

OMIM

168461

UniProt

P24385

Pathways

PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling