E2F1 antibody (pThr433)

Catalog No. ABIN1531270

The Rabbit Polyclonal anti-E2F1 antibody has been validated for WB and ELISA. It is suitable to detect E2F1 in samples from Human and Mouse.

Quick Overview for E2F1 antibody (pThr433) (ABIN1531270)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-E2F1 Antibody

Binding Specificity: AA 388-437, pThr433

Specificity:

E2F1 (Phospho-Thr433) Antibody detects endogenous levels of E2F1 only when phosphorylated at Thr433.

PhosphorylationH:T433 M:T426

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human E2F1 around the phosphorylation site of Thr433.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:5000

Comment:

Unigene-Number: Hs.654393 (NCBI Gene Symbol: E2F1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background:

Synonyms: E2F-1, PBR3, PRB-binding protein E2F-1, RBAP-1, RBBP-3, RBBP3, Retinoblastoma binding protein 3, Retinoblastoma-associated protein 1, Transcription factor E2F1

NCBI Gene Symbol: E2F1

Molecular Weight: 46 kDa

Gene ID: 1869

OMIM: 189971

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy