PPP1R12A antibody (pThr853)

Catalog No. ABIN1531349

This anti-PPP1R12A antibody is a Rabbit Polyclonal antibody detecting PPP1R12A in WB, IHC and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for PPP1R12A antibody (pThr853) (ABIN1531349)

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R12A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-PPP1R12A Antibody

Binding Specificity: AA 621-670, pThr853

Specificity:

MYPT1 (Phospho-Thr853) Antibody detects endogenous levels of MYPT1 only when phosphorylated at Thr853.

PhosphorylationH:T853 M:T852 R:T855

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MYPT1 around the phosphorylation site of Thr853.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

Comment:

Unigene-Number: Hs.49582 (NCBI Gene Symbol: PPP1R12A)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for PPP1R12A

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Alternative Name: MYPT1

Background:

Synonyms: MBS, Myosin phosphatase target subunit 1, PPP1R12A

NCBI Gene Symbol: PPP1R12A

Molecular Weight: 115 kDa

Gene ID: 4659

OMIM: 602021

UniProt: O14974

Pathways: M Phase