p300 antibody (pSer89)
Quick Overview for p300 antibody (pSer89) (ABIN1531355)
Target
See all p300 (EP300) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 55-104, pSer89
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Specificity
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p300 (Phospho-Ser89) Antibody detects endogenous levels of p300 only when phosphorylated at Ser89.
PhosphorylationH:S89 M:S89 R:S89
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Purification
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Purity
- > 95 %
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Immunogen
- The antiserum was produced against synthesized peptide derived from human p300 around the phosphorylation site of Ser89.
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Isotype
- IgG
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Application Notes
- IHC: 1:50~1:100 ELISA: 1:1000
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Comment
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Unigene-Number: Hs.517517, Hs.655211 (NCBI Gene Symbol: EP300)
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Stable at -20°C for at least 1 year.
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Expiry Date
- 12 months
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- p300 (EP300) (E1A Binding Protein P300 (EP300))
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Alternative Name
- p300
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Background
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Synonyms: E1A-associated protein p300, EP300, P300 transcription coactivator homolog
NCBI Gene Symbol: EP300
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Molecular Weight
- 264 kDa
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Gene ID
- 2033
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OMIM
- 180849
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UniProt
- Q09472
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Pathways
- p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size
Target
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