Phospholipase C gamma 2 antibody (pTyr1217)

Catalog No. ABIN1531375

The Rabbit Polyclonal anti-Phospholipase C gamma 2 antibody is suitable to detect Phospholipase C gamma 2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for Phospholipase C gamma 2 antibody (pTyr1217) (ABIN1531375)

Target: Phospholipase C gamma 2 (PLCG2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C gamma 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Phospholipase C gamma 2 Antibody

Binding Specificity: AA 1186-1235, pTyr1217

Purpose: PLC γ2 (phospho Tyr1217) Antibody

Specificity: Phospho-PLC γ2 (Y1217) Polyclonal Antibody detects endogenous levels of PLC γ2 protein only when phosphorylated at Y1217.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PLCG2 around the phosphorylation site of Tyr1217. AA range:1186-1235

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Phospholipase C gamma 2

Target: Phospholipase C gamma 2 (PLCG2)

Alternative Name: PLCG2

Background:

Synonyms: PLCG2, 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-2, Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, PLC-IV, Phospholipase C-gamma-2, PLC-gamma-2

Background: The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014],

Gene Name: PLCG2

Protein Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2

Molecular Weight: 147 kDa

Gene ID: 5336

OMIM: 600220

UniProt: P16885

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling