SYK antibody (pTyr323)

Catalog No. ABIN1531397

The Rabbit Polyclonal anti-SYK antibody (ABIN1531397) specifically detects SYK in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SYK antibody (pTyr323) (ABIN1531397)

Target: SYK (Spleen tyrosine Kinase (SYK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-SYK Antibody

Binding Specificity: AA 289-338, pTyr323

Purpose: Syk (phospho Tyr323) Antibody

Specificity: Phospho-Syk (Y323) Polyclonal Antibody detects endogenous levels of Syk protein only when phosphorylated at Y323.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human SYK around the phosphorylation site of Tyr323. AA range:289-338

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Alternative Name: SYK

Background:

Synonyms: SYK, Tyrosine-protein kinase SYK, Spleen tyrosine kinase, p72-Syk

Background: This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010],

Gene Name: SYK

Protein Name: Tyrosine-protein kinase SYK

Molecular Weight: 72 kDa

Gene ID: 6850

OMIM: 600085

UniProt: P43405

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling