Cyclin B1 antibody (pSer147)

Catalog No. ABIN1531429

This Rabbit Polyclonal antibody specifically detects Cyclin B1 in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cyclin B1 antibody (pSer147) (ABIN1531429)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Cyclin B1 Antibody

Binding Specificity: AA 121-170, pSer147

Specificity:

Cyclin B1 (Phospho-Ser147) Antibody detects endogenous levels of Cyclin B1 only when phosphorylated at Ser147.

PhosphorylationH:S147 M:S144 R:S137

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin B1 around the phosphorylation site of Ser147.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.23960 (NCBI Gene Symbol: CCNB1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1

Background:

Synonyms: CCN-2, CCNB, CCNB1, CCNB1-RS1, CYCB, CYCB1, G2/mitotic-specific cyclin B1

NCBI Gene Symbol: CCNB1

Molecular Weight: 48 kDa

Gene ID: 891

OMIM: 123836

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase