Cyclin E1 antibody (pThr77)

Catalog No. ABIN1531430

The Rabbit Polyclonal anti-Cyclin E1 antibody has been validated for ELISA and IF. It is suitable to detect Cyclin E1 in samples from Human.

Quick Overview for Cyclin E1 antibody (pThr77) (ABIN1531430)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Product Details anti-Cyclin E1 Antibody

Binding Specificity: AA 43-92, pThr77

Specificity:

Cyclin E1 (Phospho-Thr77) Antibody detects endogenous levels of Cyclin E1 only when phosphorylated at Thr77.

PhosphorylationH:T77

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin E1 around the phosphorylation site of Thr77.

Isotype: IgG

Application Details

Application Notes: IF: 1:100~1:500 ELISA: 1:10000

Comment:

Unigene-Number: Hs.244723 (NCBI Gene Symbol: CCNE1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: Cyclin E1

Background:

Synonyms: CCNE, CCNE1, G1/S-specific cyclin E1

NCBI Gene Symbol: CCNE1

Molecular Weight: 47 kDa

Gene ID: 898

OMIM: 123837

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases