PBK antibody (pThr9)

Catalog No. ABIN1531458

The Rabbit Polyclonal anti-PBK antibody (ABIN1531458) specifically detects PBK in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PBK antibody (pThr9) (ABIN1531458)

Target: PBK (PDZ Binding Kinase (PBK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PBK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PBK Antibody

Binding Specificity: AA 1-50, pThr9

Purpose: PBK (phospho Thr9) Antibody

Specificity: Phospho-PBK (T9) Polyclonal Antibody detects endogenous levels of PBK protein only when phosphorylated at T9.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PBK/TOPK around the phosphorylation site of Thr9. AA range:1-50

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PBK

Target: PBK (PDZ Binding Kinase (PBK))

Alternative Name: PBK/TOPK

Background:

Synonyms: PBK, TOPK, Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, CT84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, SPK, T-LAK cell-originated protein kinas

Background: PDZ binding kinase(PBK) Homo sapiens This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],

Gene Name: PBK

Protein Name: Lymphokine-activated killer T-cell-originated protein kinase

Molecular Weight: 36 kDa

Gene ID: 55872

OMIM: 611210

UniProt: Q96KB5