PKC delta antibody (pThr505)

Catalog No. ABIN1531463

The Rabbit Polyclonal anti-PKC delta antibody is suitable to detect PKC delta in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for PKC delta antibody (pThr505) (ABIN1531463)

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC delta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PKC delta Antibody

Binding Specificity: AA 471-520, pThr505

Purpose: PKC δ (phospho Thr507) Antibody

Specificity: Phospho-PKC δ (T507) Polyclonal Antibody detects endogenous levels of PKC δ protein only when phosphorylated at T507.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PKC delta around the phosphorylation site of Thr505. AA range:471-520

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PKC delta

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Alternative Name: PKC delta

Background:

Synonyms: PRKCD, Protein kinase C delta type, Tyrosine-protein kinase PRKCD, nPKC-delta

Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells.The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008],

Gene Name: PRKCD

Protein Name: Protein kinase C delta type

Molecular Weight: 77 kDa

Gene ID: 5580

OMIM: 176977

UniProt: Q05655

Pathways: Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism