Actin antibody (pTyr53, pTyr55)

Catalog No. ABIN1531480

This Rabbit Polyclonal antibody specifically detects Actin in IHC, IF and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Actin antibody (pTyr53, pTyr55) (ABIN1531480)

Target: Actin

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-Actin Antibody

Binding Specificity: AA 21-70, pTyr53, pTyr55

Purpose: Actin-α/γ (phospho Tyr55/53) Antibody

Specificity: Phospho-Actin-α/γ (Y55/53) Polyclonal Antibody detects endogenous levels of Actin-α/γ protein only when phosphorylated at Y55/53.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Actin-pan around the phosphorylation site of Tyr55/53. AA range:21-70

Isotype: IgG

Application Details

Application Notes: IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Actin

Target: Actin

Background:

Synonyms: ACTC1, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ACTG1, ACTB, ACTG, Actin, cytoplasmic 2, Gamma-actin, ACTG2, ACTA3, ACTL3, ACTSG, Actin, gamma-enteric smooth muscle, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin,

Background: Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others.The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008],

Gene Name: ACTC1

Protein Name: Actin alpha cardiac muscle 1

Molecular Weight: 41 kDa

Gene ID: 70, 71, 72

OMIM: 102540

UniProt: P68032