ADAM17 antibody (pThr735)

Catalog No. ABIN1531481

There is 1 publication for this product available. The Rabbit Polyclonal anti-ADAM17 antibody is suitable to detect ADAM17 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for ADAM17 antibody (pThr735) (ABIN1531481)

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ADAM17 Antibody

Binding Specificity: AA 701-750, pThr735

Purpose: TACE (phospho Thr735) Antibody

Specificity: Phospho-TACE (T735) Polyclonal Antibody detects endogenous levels of TACE protein only when phosphorylated at T735.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ADAM 17 around the phosphorylation site of Thr735. AA range:701-750

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

References for anti-ADAM17 antibody (ABIN1531481)

Bilousova, Taylor, Emirzian, Gylys, Frautschy, Cole, Teng: "Parallel age-associated changes in brain and plasma neuronal pentraxin receptor levels in a transgenic APP/PS1 rat model of Alzheimer's disease." in: Neurobiology of disease, Vol. 74, pp. 32-40, (2015) (PubMed).

Target Details for ADAM17

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Alternative Name: ADAM 17

Background:

Synonyms: ADAM17, CSVP, TACE, Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, CD antigen CD156b

Background: ADAM metallopeptidase domain 17(ADAM17) Homo sapiens This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands. The encoded protein also plays a prominent role in the activation o

Gene Name: ADAM17

Protein Name: Disintegrin and metalloproteinase domain-containing protein 17

Molecular Weight: 93 kDa

Gene ID: 6868

OMIM: 603639

UniProt: P78536

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus