PKC epsilon antibody (pSer729)

Catalog No. ABIN1531507

This Rabbit Polyclonal antibody specifically detects PKC epsilon in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PKC epsilon antibody (pSer729) (ABIN1531507)

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC epsilon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-PKC epsilon Antibody

Binding Specificity: AA 688-737, pSer729

Purpose: PKC ε (phospho Ser729) Antibody

Specificity: Phospho-PKC ε (S729) Polyclonal Antibody detects endogenous levels of PKC ε protein only when phosphorylated at S729.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PKC epsilon around the phosphorylation site of Ser729. AA range:688-737

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,IHC 1:50-300, ELISA 2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PKC epsilon

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Alternative Name: PKC epsilon

Background:

Synonyms: PRKCE, PKCE, Protein kinase C epsilon type, nPKC-epsilon

Background: protein kinase C epsilon(PRKCE) Homo sapiens Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macro

Gene Name: PRKCE

Protein Name: Protein kinase C epsilon type

Molecular Weight: 83 kDa

Gene ID: 5581

OMIM: 176975

UniProt: Q02156

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling