CRYAB antibody (pSer59)

Catalog No. ABIN1531574

The Rabbit Polyclonal anti-CRYAB antibody is suitable to detect CRYAB in samples from Human, Rat and Mouse. It has been validated for IHC, ELISA and IF.

Quick Overview for CRYAB antibody (pSer59) (ABIN1531574)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRYAB antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CRYAB Antibody

Binding Specificity: AA 31-80, pSer59

Purpose: Crystallin-αB (phospho Ser59) Antibody

Specificity: Phospho-Crystallin-αB (S59) Polyclonal Antibody detects endogenous levels of Crystallin-αB protein only when phosphorylated at S59.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CRYAB/Crystallin-alpha-B around the phosphorylation site of Ser59. AA range:31-80

Isotype: IgG

Application Details

Application Notes: IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: CRYAB/Crystallin-alpha-B

Background:

Synonyms: CRYAB, CRYA2, Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component

Background: Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone, instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits, the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distin

Gene Name: CRYAB

Protein Name: Alpha-crystallin B chain

Molecular Weight: 20 kDa

Gene ID: 1410

OMIM: 123590

UniProt: P02511