Aurora Kinase B antibody (pThr232)

Catalog No. ABIN1531642

This Rabbit Polyclonal antibody specifically detects Aurora Kinase B in IHC, WB, ELISA and IF. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for Aurora Kinase B antibody (pThr232) (ABIN1531642)

Target: Aurora Kinase B (AURKB)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-Aurora Kinase B Antibody

Binding Specificity: AA 198-247, pThr232

Purpose: ARK-2 (phospho Thr232) Antibody

Specificity: Phospho-ARK-2 (T232) Polyclonal Antibody detects endogenous levels of ARK-2 protein only when phosphorylated at T232.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human AurB around the phosphorylation site of Thr232. AA range:198-247

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: AurB

Background:

Synonyms: AURKB, AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5, Aurora kinase B, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, ARK-2, Aurora-related kinase 2, STK-1, Serine/threonine-protein kinase 12

Background: This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015],

Gene Name: AURKB

Protein Name: Aurora kinase B,Aurora B

Molecular Weight: 39 kDa

Gene ID: 9212

OMIM: 604970

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades