HRS (HRS) (AA 301-350), (pTyr334) antibody

Catalog No. ABIN1531658

The Rabbit Polyclonal anti-HRS antibody is suitable to detect HRS in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for HRS (HRS) (AA 301-350), (pTyr334) antibody (ABIN1531658)

Target: HRS

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details

Binding Specificity: AA 301-350, pTyr334

Purpose: Hrs (phospho Tyr334) Antibody

Specificity: Phospho-Hrs (Y334) Polyclonal Antibody detects endogenous levels of Hrs protein only when phosphorylated at Y334.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HRS around the phosphorylation site of Tyr334. AA range:301-350

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details

Target: HRS

Alternative Name: HRS

Background:

Synonyms: HGS, HRS, Hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, Protein pp110

Background: The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010],

Gene Name: HGS

Protein Name: Hepatocyte growth factor-regulated tyrosine kinase substrate

Molecular Weight: 86 kDa

Gene ID: 9146

OMIM: 604375

UniProt: O14964

Pathways: CXCR4-mediated Signaling Events, EGFR Downregulation