HRS (HRS) (pTyr334) antibody

Catalog No. ABIN7225838

The Rabbit Polyclonal anti-HRS antibody is suitable to detect HRS in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for HRS (HRS) (pTyr334) antibody (ABIN7225838)

Target: HRS

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details

Binding Specificity: pTyr334

Purpose: Rabbit Anti-Hrs (phospho Tyr334) Polyclonal Antibody

Specificity: Phospho-Hrs (Y334) Polyclonal Antibody detects endogenous levels of Hrs protein only when phosphorylated at Y334.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Hrs Phospho-Tyr334

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: HRS

Alternative Name: Hrs

Background: HGS, HRS, Hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, Protein pp110The protein encoded by HGS (hepatocyte growth factor-regulated tyrosine kinase substrate) regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation.

Molecular Weight: 86kD

Gene ID: 9146

UniProt: O14964

Pathways: CXCR4-mediated Signaling Events, EGFR Downregulation