LEF1 antibody (pSer42)

Catalog No. ABIN1531685

The Rabbit Polyclonal anti-LEF1 antibody has been validated for WB and ELISA. It is suitable to detect LEF1 in samples from Human, Mouse and Rat.

Quick Overview for LEF1 antibody (pSer42) (ABIN1531685)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-LEF1 Antibody

Binding Specificity: AA 8-57, pSer42

Specificity:

LEF-1 (Phospho-Ser42) Antibody detects endogenous levels of LEF-1 only when phosphorylated at Ser42.

PhosphorylationH:S42 M:S40 R:S40

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human LEF-1 around the phosphorylation site of Ser42.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:5000

Comment:

Unigene-Number: Hs.555947 (NCBI Gene Symbol: LEF1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF-1

Background:

Synonyms: LEF1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha

NCBI Gene Symbol: LEF1

Molecular Weight: 44 kDa

Gene ID: 51176

OMIM: 153245

UniProt: Q9UJU2

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding