RAD51 antibody (pTyr315)

Catalog No. ABIN1531695

The Rabbit Polyclonal anti-RAD51 antibody (ABIN1531695) specifically detects RAD51 in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for RAD51 antibody (pTyr315) (ABIN1531695)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD51 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-RAD51 Antibody

Binding Specificity: AA 281-330, pTyr315

Purpose: Rad51 (phospho Tyr315) Antibody

Specificity: Phospho-Rad51 (Y315) Polyclonal Antibody detects endogenous levels of Rad51 protein only when phosphorylated at Y315.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human RAD51 around the phosphorylation site of Tyr315. AA range:281-330

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Alternative Name: RAD51

Background:

Synonyms: RAD51, RAD51A, RECA, DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A

Background: The protein encoded byThis gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009],

Gene Name: RAD51

Protein Name: DNA repair protein RAD51 homolog 1

Molecular Weight: 36 kDa

Gene ID: 5888

OMIM: 114480

UniProt: Q06609

Pathways: DNA Damage Repair