MAP2K4 antibody (pSer257)

Catalog No. ABIN1531699

This Rabbit Polyclonal antibody specifically detects MAP2K4 in IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MAP2K4 antibody (pSer257) (ABIN1531699)

Target: MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Product Details anti-MAP2K4 Antibody

Binding Specificity: AA 223-272, pSer257

Specificity:

SEK1/MKK4/JNKK1 (Phospho-Ser257) Antibody detects endogenous levels of SEK1/MKK4/JNKK1 only when phosphorylated at Ser257.

PhosphorylationH:S257 M:S255 R:S135

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human SEK1/MKK4/JNKK1 around the phosphorylation site of Ser257.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:40000

Comment:

Unigene-Number: Hs.514681 (NCBI Gene Symbol: MAP2K4)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for MAP2K4

Target: MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

Alternative Name: SEK1/MKK4/JNKK1

Background:

Synonyms: c-Jun N- terminal kinase kinase 1, C-JUN N-terminal kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 4, JNK activating kinase 1, JNK kinase 1, JNKK, JNKK 1, JNKK1, kinase SEK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK

NCBI Gene Symbol: MAP2K4

Molecular Weight: 44 kDa

Gene ID: 6416

OMIM: 601335

UniProt: P45985

Pathways: MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling