NCF1 antibody (pSer328)

Catalog No. ABIN1531718

There is 1 publication for this product available. The Rabbit Polyclonal anti-NCF1 antibody is suitable to detect NCF1 in samples from Human, Mouse, Rat and Cow. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for NCF1 antibody (pSer328) (ABIN1531718)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-NCF1 Antibody

Binding Specificity: AA 301-350, pSer328

Purpose: p47-phox (phospho Ser328) Antibody

Specificity: Phospho-p47-phox (S328) Polyclonal Antibody detects endogenous levels of p47-phox protein only when phosphorylated at S328.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of Ser328. AA range:301-350

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:10000. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

References for anti-NCF1 antibody (ABIN1531718)

Kröller-Schön, Steven, Kossmann, Scholz, Daub, Oelze, Xia, Hausding, Mikhed, Zinßius, Mader, Stamm, Treiber, Scharffetter-Kochanek, Li, Schulz, Wenzel, Münzel, Daiber: "Molecular mechanisms of the crosstalk between mitochondria and NADPH oxidase through reactive oxygen species - studies in white blood cells and in animal models." in: Antioxidants & redox signaling, (2013) (PubMed).

Target Details for NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name: Neutrophil Cytosol Factor 1

Background:

Synonyms: NCF1, NOXO2, SH3PXD1A, Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3

Background: The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008],

Gene Name: NCF1

Protein Name: Neutrophil cytosol factor 1

Molecular Weight: 44 kDa

Gene ID: 653361

OMIM: 233700

UniProt: P14598

Pathways: PI3K-Akt Signaling