NPM1 antibody (pThr234)

Catalog No. ABIN1531720

This Rabbit Polyclonal antibody specifically detects NPM1 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NPM1 antibody (pThr234) (ABIN1531720)

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NPM1 Antibody

Binding Specificity: AA 201-250, pThr234

Specificity:

NPM (Phospho-Thr234) Antibody detects endogenous levels of NPM only when phosphorylated at Thr234.

PhosphorylationH:T234 M:T232 R:T232

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr234.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:1000

Comment:

Unigene-Number: Hs.557550, Hs.654469, Hs.654583 (NCBI Gene Symbol: NPM1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name: NPM

Background:

Synonyms: B23.1, NPM isoform 1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, nucleophosmin, nucleophosmin (nucleolar phosphoprotein B23, numatrin), Numatrin

NCBI Gene Symbol: NPM1

Molecular Weight: 32 kDa

Gene ID: 4869

OMIM: 164040

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly