NCF1 antibody (pSer370)

Catalog No. ABIN1531722

This anti-NCF1 antibody is a Rabbit Polyclonal antibody detecting NCF1 in ELISA and IHC. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for NCF1 antibody (pSer370) (ABIN1531722)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCF1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-NCF1 Antibody

Binding Specificity: AA 341-390, pSer370

Specificity:

p47 phox (Phospho-Ser370) Antibody detects endogenous levels of p47 phox only when phosphorylated at Ser370.

PhosphorylationH:S370 M:S370 R:S369

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human p47 phox around the phosphorylation site of Ser370.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:5000

Comment:

Unigene-Number: Hs.647047, Hs.655201 (NCBI Gene Symbol: NCF1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-NCF1 antibody (ABIN1531722)

Lee, Lee, Park, Jang, Kim, Kim: "Anti-Inflammatory and Antioxidant Mechanism of Tangeretin in Activated Microglia." in: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, (2016) (PubMed).

Lee, Ko, Jeong, Park, Kim: "β-Lapachone suppresses neuroinflammation by modulating the expression of cytokines and matrix metalloproteinases in activated microglia." in: Journal of neuroinflammation, Vol. 12, pp. 133, (2015) (PubMed).

Target Details for NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name: p47 phox

Background:

Synonyms: 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil cytosol factor 1, Neutrophil cytosolic factor 1, Neutrophil NADPH oxidase factor 1, P47 phox, p47-phox

NCBI Gene Symbol: NCF1

Molecular Weight: 44 kDa

Gene ID: 653361

OMIM: 233700

UniProt: P14598

Pathways: PI3K-Akt Signaling