S1PR1 antibody (pSer1, pThr236)

Catalog No. ABIN1531727

The Rabbit Polyclonal anti-S1PR1 antibody is suitable to detect S1PR1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IF.

Quick Overview for S1PR1 antibody (pSer1, pThr236) (ABIN1531727)

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-S1PR1 Antibody

Binding Specificity: AA 206-255, pSer1, pThr236

Purpose: EDG-1 (phospho Thr236) Antibody

Specificity: Phospho-EDG-1 (T236) Polyclonal Antibody detects endogenous levels of EDG-1 protein only when phosphorylated at T236.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human S1P Receptor EDG1 around the phosphorylation site of Thr236. AA range:206-255

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000 IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for S1PR1

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Alternative Name: S1P Receptor EDG1

Background:

Synonyms: S1PR1, CHEDG1, EDG1, Sphingosine 1-phosphate receptor 1, S1P receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, Sphingosine 1-phosphate receptor Edg-1, S1P receptor Edg-1, CD antigen CD363

Background: The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],

Gene Name: S1PR1

Protein Name: Sphingosine 1-phosphate receptor 1

Molecular Weight: 42 kDa

Gene ID: 1901

OMIM: 601974

UniProt: P21453

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2