TERT antibody (pSer227)

Catalog No. ABIN1531729

This Rabbit Polyclonal antibody specifically detects TERT in ELISA and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for TERT antibody (pSer227) (ABIN1531729)

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TERT antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Product Details anti-TERT Antibody

Binding Specificity: AA 196-245, pSer227

Purpose: TERT (phospho Ser227) Antibody

Specificity: Phospho-TERT (S227) Polyclonal Antibody detects endogenous levels of TERT protein only when phosphorylated at S227.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Telomerase around the phosphorylation site of Ser227. AA range:196-245

Isotype: IgG

Application Details

Application Notes: IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for TERT

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Alternative Name: Telomerase

Background:

Synonyms: TERT, EST2, TCS1, TRT, Telomerase reverse transcriptase, HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2

Background: Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified, the full-length sequence of some variants has not been determined. Alternative sp

Gene Name: TERT

Protein Name: Telomerase reverse transcriptase

Molecular Weight: 126 kDa

Gene ID: 7015

OMIM: 127550

UniProt: O14746

Pathways: Telomere Maintenance