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CHEK2 antibody (pSer516)

The Rabbit Polyclonal anti-CHEK2 antibody has been validated for WB and ELISA. It is suitable to detect CHEK2 in samples from Human.
Catalog No. ABIN1531802

Quick Overview for CHEK2 antibody (pSer516) (ABIN1531802)

Target

See all CHEK2 Antibodies
CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This CHEK2 antibody is un-conjugated

Application

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Western Blotting (WB), ELISA
  • Binding Specificity

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    AA 486-535, pSer516

    Specificity

    Chk2 (Phospho-Ser516) Antibody detects endogenous levels of Chk2 only when phosphorylated at Ser516.

    PhosphorylationH:S516

    Purification

    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human Chk2 around the phosphorylation site of Ser516.

    Isotype

    IgG
  • Application Notes

    WB: 1:500~1:1000 ELISA: 1:10000

    Comment

    Unigene-Number: Hs.291363, Hs.505297 (NCBI Gene Symbol: CHEK2)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable at -20°C for at least 1 year.

    Expiry Date

    12 months
  • Target

    CHEK2 (Checkpoint Kinase 2 (CHEK2))

    Alternative Name

    Chk2

    Background

    Synonyms: Cds1, CHEK2, CHK2, Chk2, CHK2 checkpoint homolog (S. pombe), kinase Chk2, RAD53

    NCBI Gene Symbol: CHEK2

    Molecular Weight

    60 kDa

    Gene ID

    11200

    OMIM

    176807

    UniProt

    O96017

    Pathways

    p53 Signaling, Apoptosis, Cell Division Cycle
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