CHEK2 antibody (pThr68)

Catalog No. ABIN1531803

This anti-CHEK2 antibody is a Rabbit Polyclonal antibody detecting CHEK2 in WB, ELISA and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for CHEK2 antibody (pThr68) (ABIN1531803)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-CHEK2 Antibody

Binding Specificity: AA 35-84, pThr68

Specificity:

Chk2 (Phospho-Thr68) Antibody detects endogenous levels of Chk2 only when phosphorylated at Thr68.

PhosphorylationH:T68 M:T77 R:T76

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Chk2 around the phosphorylation site of Thr68.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.291363, Hs.505297 (NCBI Gene Symbol: CHEK2)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: Chk2

Background:

Synonyms: Cds1, CHEK2, CHK2, Chk2, CHK2 checkpoint homolog (S. pombe), kinase Chk2, RAD53

NCBI Gene Symbol: CHEK2

Molecular Weight: 60 kDa

Gene ID: 11200

OMIM: 176807

UniProt: O96017

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle