Cortactin antibody (pTyr421)

Catalog No. ABIN1531808

The Rabbit Polyclonal anti-Cortactin antibody has been validated for WB and ELISA. It is suitable to detect Cortactin in samples from Human, Mouse and Rat.

Quick Overview for Cortactin antibody (pTyr421) (ABIN1531808)

Target: Cortactin (CTTN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cortactin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Cortactin Antibody

Binding Specificity: AA 388-437, pTyr421

Specificity:

Cortactin (Phospho-Tyr421) Antibody detects endogenous levels of Cortactin only when phosphorylated at Tyr421.

PhosphorylationH:Y421 M:Y421 R:Y384

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Cortactin around the phosphorylation site of Tyr421.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:20000

Comment:

Unigene-Number: Hs.596164 (NCBI Gene Symbol: CTTN)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Cortactin

Target: Cortactin (CTTN)

Alternative Name: Cortactin

Background:

Synonyms: Src substrate cortactin, Amplaxin, EMS1, CTTN, EMS1

NCBI Gene Symbol: CTTN

Molecular Weight: 61 kDa

Gene ID: 2017

OMIM: 164765

UniProt: Q14247

Pathways: MAPK Signaling