HSF1 antibody (pSer303)

Catalog No. ABIN1531865

The Rabbit Polyclonal anti-HSF1 antibody is suitable to detect HSF1 in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for HSF1 antibody (pSer303) (ABIN1531865)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-HSF1 Antibody

Binding Specificity: AA 270-319, pSer303

Purpose: HSF1 (phospho Ser303) Antibody

Specificity: Phospho-HSF1 (S303) Polyclonal Antibody detects endogenous levels of HSF1 protein only when phosphorylated at S303.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HSF1 around the phosphorylation site of Ser303. AA range:270-319

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1

Background:

Synonyms: HSF1, HSTF1, Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1

Background: heat shock transcription factor 1(HSF1) Homo sapiens The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phsphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Aug 2016],

Gene Name: HSF1

Protein Name: Heat shock factor protein 1

Molecular Weight: 57 kDa

Gene ID: 3297

OMIM: 140580

UniProt: Q00613