JAK2 antibody (pTyr221)

Catalog No. ABIN1531883

The Rabbit Polyclonal anti-JAK2 antibody has been validated for WB, IHC and ELISA. It is suitable to detect JAK2 in samples from Human, Rat and Mouse.

Quick Overview for JAK2 antibody (pTyr221) (ABIN1531883)

Target: JAK2 (Janus Kinase 2 (JAK2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-JAK2 Antibody

Binding Specificity: AA 191-240, pTyr221

Specificity:

JAK2 (Phospho-Tyr221) Antibody detects endogenous levels of JAK2 only when phosphorylated at Tyr221.

PhosphorylationH:Y221 M:Y221 R:Y221

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human JAK2 around the phosphorylation site of Tyr221.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.656213 (NCBI Gene Symbol: JAK2)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for JAK2

Target: JAK2 (Janus Kinase 2 (JAK2))

Alternative Name: JAK2

Background:

Synonyms: JAK-2, JAK2, Janus kinase 2, kinase Jak2

NCBI Gene Symbol: JAK2

Molecular Weight: 130 kDa

Gene ID: 3717

OMIM: 147796

UniProt: O60674

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response