NF-kB p65 antibody (pSer529)

Catalog No. ABIN1531916

The Rabbit Polyclonal anti-NF-kB p65 antibody has been validated for WB, IHC and ELISA. It is suitable to detect NF-kB p65 in samples from Human and Mouse.

Quick Overview for NF-kB p65 antibody (pSer529) (ABIN1531916)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NF-kB p65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-NF-kB p65 Antibody

Binding Specificity: AA 496-545, pSer529

Specificity:

NF-kappaB p65 (Phospho-Ser529) Antibody detects endogenous levels of NF-kappaB p65 only when phosphorylated at Ser529.

PhosphorylationH:S529 M:S528

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser529.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.502875 (NCBI Gene Symbol: RELA)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: NF-KappaB p65

Background:

Synonyms: NFKB3, nuclear factor NF-kappa-B p65 subunit, p65, RELA, TF65, transcription factor p65

NCBI Gene Symbol: RELA

Molecular Weight: 60 kDa

Gene ID: 5970

OMIM: 164014

UniProt: Q04206

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins