Paxillin antibody (pTyr31)

Catalog No. ABIN1531947

The Rabbit Polyclonal anti-Paxillin antibody (ABIN1531947) specifically detects Paxillin in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Paxillin antibody (pTyr31) (ABIN1531947)

Target: Paxillin (PXN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Paxillin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Paxillin Antibody

Binding Specificity: AA 15-64, pTyr31

Purpose: Paxillin (phospho Tyr31) Antibody

Specificity: Phospho-Paxillin (Y31) Polyclonal Antibody detects endogenous levels of Paxillin protein only when phosphorylated at Y31.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Paxillin around the phosphorylation site of Tyr31. AA range:15-64

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Paxillin

Target: Paxillin (PXN)

Alternative Name: Paxillin

Background:

Synonyms: PXN, Paxillin

Background: This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011],

Gene Name: PXN

Protein Name: Paxillin

Molecular Weight: 64 kDa

Gene ID: 5829

OMIM: 602505

UniProt: P49023

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling