MLK1/2 antibody (pThr266, pThr312)

Catalog No. ABIN1532062

The Rabbit Polyclonal anti-MLK1/2 antibody is suitable to detect MLK1/2 in samples from Human and Mouse. It has been validated for ELISA, IHC and IF.

Quick Overview for MLK1/2 antibody (pThr266, pThr312) (ABIN1532062)

Target: MLK1/2

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLK1/2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MLK1/2 Antibody

Binding Specificity: AA 281-330, pThr266, pThr312

Purpose: MLK1/2 (phospho Thr312/266) Antibody

Specificity: Phospho-MLK1/2 (T312/266) Polyclonal Antibody detects endogenous levels of MLK1/2 protein only when phosphorylated at T312/266.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MLK1/2 around the phosphorylation site of Thr312/266. AA range:281-330

Isotype: IgG

Application Details

Application Notes: IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MLK1/2

Target: MLK1/2

Background:

Synonyms: MAP3K9, MLK1, PRKE1, Mitogen-activated protein kinase kinase kinase 9, Mixed lineage kinase 1, MAP3K10, MLK2, MST, Mitogen-activated protein kinase kinase kinase 10, Mixed lineage kinase 2, Protein kinase MST

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,function:Activates the JUN N-terminal pathway.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH3 domain.,subunit:Homodimer.,tissue specificity:Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.,

Gene Name: MAP3K9/MAP3K10

Protein Name: Mitogen-activated protein kinase kinase kinase 9/10

Molecular Weight: 121 kDa

Gene ID: 4293, 4294

OMIM: 600136

UniProt: P80192