WEE1 antibody (pSer53)

Catalog No. ABIN1532086

The Rabbit Polyclonal anti-WEE1 antibody is suitable to detect WEE1 in samples from Human, Mouse and Rat. It has been validated for ELISA and IHC.

Quick Overview for WEE1 antibody (pSer53) (ABIN1532086)

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-WEE1 Antibody

Binding Specificity: AA 19-68, pSer53

Specificity:

WEE1 (Phospho-Ser53) Antibody detects endogenous levels of WEE1 only when phosphorylated at Ser53.

PhosphorylationH:S53 M:S52 R:S52

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human WEE1 around the phosphorylation site of Ser53.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.249441 (NCBI Gene Symbol: WEE1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Alternative Name: WEE1

Background:

Synonyms: WEE1hu, Wee1-like protein kinase, kinase WEE1

NCBI Gene Symbol: WEE1

Molecular Weight: 71 kDa

Gene ID: 7465

OMIM: 193525

UniProt: P30291

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase