ITCH antibody (pTyr420)

Catalog No. ABIN1532110

Product Details anti-ITCH Antibody

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Binding Specificity: AA 386-435, pTyr420

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITCH antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Specificity:

ITCH (Phospho-Tyr420) Antibody detects endogenous levels of ITCH only when phosphorylated at Tyr420.

PhosphorylationH:Y420 M:Y381

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ITCH around the phosphorylation site of Tyr420.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.632272 (NCBI Gene Symbol: ITCH)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH (ITCH Products)

Background:

Synonyms: AIF4, AIP4, atrophin-1-interacting protein 4, Itchy homolog E3 ubiquitin protein ligase, NAPP1, NFE2-associated polypeptide 1, ubiquitin protein ligase ITCH

NCBI Gene Symbol: ITCH

Molecular Weight: 102 kDa

Gene ID: 83737

OMIM: 606409

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events