Cyclin D2 antibody (pThr280)

Catalog No. ABIN1532139

This Rabbit Polyclonal antibody specifically detects Cyclin D2 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cyclin D2 antibody (pThr280) (ABIN1532139)

Target: Cyclin D2 (CCND2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Cyclin D2 Antibody

Binding Specificity: AA 240-289, pThr280

Purpose: Cyclin D2 (phospho Thr280) Antibody

Specificity: Phospho-Cyclin D2 (T280) Polyclonal Antibody detects endogenous levels of Cyclin D2 protein only when phosphorylated at T280.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin D2 around the phosphorylation site of Thr280. AA range:240-289

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Cyclin D2

Target: Cyclin D2 (CCND2)

Alternative Name: Cyclin D2

Background:

Synonyms: CCND2, G1/S-specific cyclin-D2

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencep

Gene Name: CCND2

Protein Name: G1/S-specific cyclin-D2

Molecular Weight: 33 kDa

Gene ID: 894

OMIM: 123833

UniProt: P30279

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases