Phospholipase D2 antibody (pTyr169)

Catalog No. ABIN1532165

This Rabbit Polyclonal antibody specifically detects Phospholipase D2 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Phospholipase D2 antibody (pTyr169) (ABIN1532165)

Target: Phospholipase D2 (PLD2)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase D2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Phospholipase D2 Antibody

Binding Specificity: AA 136-185, pTyr169

Purpose: PC-PLD2 (phospho Tyr169) Antibody

Specificity: Phospho-PC-PLD2 (Y169) Polyclonal Antibody detects endogenous levels of PC-PLD2 protein only when phosphorylated at Y169.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PLD2 around the phosphorylation site of Tyr169. AA range:136-185

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Phospholipase D2

Target: Phospholipase D2 (PLD2)

Alternative Name: PLD2

Background:

Synonyms: PLD2, Phospholipase D2, PLD 2, hPLD2, Choline phosphatase 2, PLD1C, Phosphatidylcholine-hydrolyzing phospholipase D2

Background: The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011],

Gene Name: PLD2

Protein Name: Phospholipase D2

Molecular Weight: 105 kDa

Gene ID: 5338

OMIM: 602384

UniProt: O14939

Pathways: RTK Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling