TAL1 antibody (pSer122)

Catalog No. ABIN1532171

This Rabbit Polyclonal antibody specifically detects TAL1 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for TAL1 antibody (pSer122) (ABIN1532171)

Target: TAL1 (T-Cell Acute Lymphocytic Leukemia 1 (TAL1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TAL1 Antibody

Binding Specificity: AA 96-145, pSer122

Purpose: TAL1 (phospho Ser122) Antibody

Specificity: Phospho-TAL1 (S122) Polyclonal Antibody detects endogenous levels of TAL1 protein only when phosphorylated at S122.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human TAL-1 around the phosphorylation site of Ser122. AA range:96-145

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for TAL1

Target: TAL1 (T-Cell Acute Lymphocytic Leukemia 1 (TAL1))

Alternative Name: TAL-1

Background:

Synonyms: TAL1, BHLHA17, SCL, TCL5, T-cell acute lymphocytic leukemia protein 1, TAL-1, Class A basic helix-loop-helix protein 17, bHLHa17, Stem cell protein, T-cell leukemia/lymphoma protein 5

Background: alternative products:The splicing pattern is cell-lineage dependent,disease:A chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). Translocation t(1,14)(p32,q11) with T-cell receptor alpha chain (TCRA) genes.,domain:The helix-loop-helix domain is necessary and sufficient for the interaction with DRG1.,function:Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation.,PTM:Phosphorylated on serine residues. Phosphorylation of Ser-122 is strongly stimulated by hypoxia.,PTM:Ubiquitinated, subsequent to hypoxia-dependent phosphorylation of Ser-122, ubiquitination targets the protein for rapid degradation via the ubiquitin system. This process may be characteristic for microvascular endothelial cells, since it could not be observed in large vessel endothelial cells.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Forms heterodimers with TCF3. Binds to the LIM domain containing protein LMO2 and to DRG1. Can assemble in a complex with LDB1 and LMO2. Component of a TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3.,tissue specificity:Leukemic stem cell.,

Gene Name: TAL1

Protein Name: T-cell acute lymphocytic leukemia protein 1

Molecular Weight: 34 kDa

Gene ID: 6886

OMIM: 187040

UniProt: P17542

Pathways: Stem Cell Maintenance