14-3-3 zeta antibody (AA 24-73)

Catalog No. ABIN1532187

The Rabbit Polyclonal anti-14-3-3 zeta antibody is suitable to detect 14-3-3 zeta in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IF and IP.

Quick Overview for 14-3-3 zeta antibody (AA 24-73) (ABIN1532187)

Target: 14-3-3 zeta (YWHAZ)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 zeta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-14-3-3 zeta Antibody

Binding Specificity: AA 24-73

Purpose: 14-3-3 ζ Antibody

Specificity: 14-3-3 ζ Polyclonal Antibody detects endogenous levels of 14-3-3 ζ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta. AA range:24-73

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 μg:mg lysate. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for 14-3-3 zeta

Target: 14-3-3 zeta (YWHAZ)

Alternative Name: 14-3-3 zeta

Background:

Synonyms: YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1

Background: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],

Gene Name: YWHAZ

Protein Name: 14-3-3 protein zeta/delta

Molecular Weight: 27 kDa

Gene ID: 7534

OMIM: 601288

UniProt: P63104

Pathways: Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location