Cyclin B1 antibody (AA 91-140)

Catalog No. ABIN1532242

This Rabbit Polyclonal antibody specifically detects Cyclin B1 in WB, IHC, IF and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cyclin B1 antibody (AA 91-140) (ABIN1532242)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-Cyclin B1 Antibody

Binding Specificity: AA 91-140

Purpose: Cyclin B1 Antibody

Specificity: Cyclin B1 Polyclonal Antibody detects endogenous levels of Cyclin B1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin B1. AA range:91-140

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1

Background:

Synonyms: CCNB1, CCNB, G2/mitotic-specific cyclin-B1

Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008],

Gene Name: CCNB1

Protein Name: G2/mitotic-specific cyclin-B1

Molecular Weight: 48 kDa

Gene ID: 891

OMIM: 123836

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase