RAD17 antibody (AA 621-670)

Catalog No. ABIN1532259

The Rabbit Polyclonal anti-RAD17 antibody is suitable to detect RAD17 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for RAD17 antibody (AA 621-670) (ABIN1532259)

Target: RAD17

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-RAD17 Antibody

Binding Specificity: AA 621-670

Purpose: Rad17 Antibody

Specificity: Rad17 Polyclonal Antibody detects endogenous levels of Rad17 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human RAD17. AA range:621-670

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for RAD17

Target: RAD17

Alternative Name: RAD17

Background:

Synonyms: RAD17, R24L, Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog

Background: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, h

Gene Name: RAD17

Protein Name: Cell cycle checkpoint protein RAD17

Molecular Weight: 77 kDa

Gene ID: 5884

OMIM: 603139

UniProt: O75943