PRAS40 antibody (AA 207-256)

Catalog No. ABIN1532316

The Rabbit Polyclonal anti-PRAS40 antibody is suitable to detect PRAS40 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for PRAS40 antibody (AA 207-256) (ABIN1532316)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAS40 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PRAS40 Antibody

Binding Specificity: AA 207-256

Purpose: PRAS40 Antibody

Specificity: PRAS40 Polyclonal Antibody detects endogenous levels of PRAS40 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Akt1 S1. AA range:207-256

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Alternative Name: Akt1 S1

Background:

Synonyms: AKT1S1, PRAS40, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate

Background: AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008],

Gene Name: AKT1S1

Protein Name: Proline-rich AKT1 substrate 1

Molecular Weight: 27 kDa

Gene ID: 84335

OMIM: 610221

UniProt: Q96B36

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect