MITF antibody (AA 151-200)

Catalog No. ABIN1532362

The Rabbit Polyclonal anti-MITF antibody (ABIN1532362) specifically detects MITF in WB, IHC, IF and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for MITF antibody (AA 151-200) (ABIN1532362)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-MITF Antibody

Binding Specificity: AA 151-200

Purpose: MITF Antibody

Specificity: MITF Polyclonal Antibody detects endogenous levels of MITF protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MITF. AA range:151-200

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF

Background:

Synonyms: MITF, BHLHE32, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32

Background: This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],

Gene Name: MITF

Protein Name: Microphthalmia-associated transcription factor

Molecular Weight: 58 kDa

Gene ID: 4286

OMIM: 103470

UniProt: O75030

Pathways: Chromatin Binding