PRKACA/PRKACB antibody (AA 166-215)

Catalog No. ABIN1532391

The Rabbit Polyclonal anti-PRKACA/PRKACB antibody is suitable to detect PRKACA/PRKACB in samples from Human, Mouse, Rat and Pig. It has been validated for WB, IHC, IF and ELISA.

Quick Overview for PRKACA/PRKACB antibody (AA 166-215) (ABIN1532391)

Target: PRKACA/PRKACB (PRKACA/B) (Protein Kinase A, alpha/beta (PRKACA/B))

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKACA/PRKACB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-PRKACA/PRKACB Antibody

Binding Specificity: AA 166-215

Purpose: PKAα/β/γ cat Antibody

Specificity: PKAα/β/γ cat Polyclonal Antibody detects endogenous levels of PKAα/β/γ cat protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PKA alpha/beta CAT. AA range:166-215

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PRKACA/PRKACB

Target: PRKACA/PRKACB (PRKACA/B) (Protein Kinase A, alpha/beta (PRKACA/B))

Alternative Name: PKA alpha/beta CAT

Background:

Synonyms: PRKACA; PKACA; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACB; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PRKACG; cAMP-dependent protein kinase catalytic subunit gamma; PKA C-gamma

Background: This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Altern

Gene Name: PRKACA/PRKACB

Protein Name: cAMP-dependent protein kinase catalytic subunit alpha/beta

Molecular Weight: 40 kDa

Gene ID: 5566, 5567

OMIM: 601639

UniProt: P17612