DAP Kinase 1 antibody (AA 274-323)

Catalog No. ABIN1532590

The Rabbit Polyclonal anti-DAP Kinase 1 antibody is suitable to detect DAP Kinase 1 in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for DAP Kinase 1 antibody (AA 274-323) (ABIN1532590)

Target: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAP Kinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-DAP Kinase 1 Antibody

Binding Specificity: AA 274-323

Purpose: DAPK1 Antibody

Specificity: DAPK1 Polyclonal Antibody detects endogenous levels of DAPK1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human DAPK1. AA range:274-323

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for DAP Kinase 1

Target: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Alternative Name: DAPK1

Background:

Synonyms: DAPK1, DAPK, Death-associated protein kinase 1, DAP kinase 1

Background: Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],

Gene Name: DAPK1

Protein Name: Death-associated protein kinase 1

Molecular Weight: 160 kDa

Gene ID: 1612

OMIM: 600831

UniProt: P53355

Pathways: MAPK Signaling, Interferon-gamma Pathway